Anti-Notch-1 (9930111A19RIK, hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOT

Notch-1 (so named for "notches" in fly wings, also TAN-1) is a 300kD, 2538aa member of the Notch family of type I TM glycoproteins. It is associated with gene activation in both embryos and adults. It undergoes Golgi processing to generate a heterodimer composed of a 180-200 kD disulfide-linked extracellular domain (aa18-1664), and a 120kD membrane-bound segment (aa1665-2556) that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Human Notch-1 ECD (aa19-526), including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat Notch-1, and 60% aa identity with human Notch-2 and Notch-3. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.