Anti-Notch 1 (Notch1, Notch-1, hN1, LIN12, Lin-12, Neurogenic Locus Notch Homolog Protein 1, NOTC1,

Notch 1 is initially synthesized as inactive precursor molecule. During maturation, the molecule is cleaved at its extracellular domain. Upon binding to a ligand such as Delta1, a C-terminal Notch 1 fragment is released and further cleaved. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. Notch 1 is mostly found in fetal spleen, brain stem and lung tissues and adult lymphoid tissues. Applications: Suitable for use in Western Blot. Other applications not tested. , Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.