Anti-CD274 (CD274 Molecule, B7 Homolog 1, B7H1, B7-H1, B7-H, Programmed Cell Death 1 Ligand 1, PDCD1

CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection. CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma is associated with poor prognosis. Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival. Gamma Interferon and PHA can up regulate CD274 expression on T cells. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (80ul), Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5ug/ml PHA., 2. Five x10e5 cells/tube were washed and pre-incubated for 5 minutes with 20ul of 0.25mg/ml human IgG (to block non-specific binding) after which they were incubated 45 minutes on ice with 80ul of C2549-22P2 at 10ug/ml. , 3. Cells were washed twice and incubated with S7973-88P, after which they were washed twice, fixed and analyzed by FACS using a lymphoid gate. , 4. Cells stained positive with a mean shift of 1.14 log10 fluorescent units when compared to a Mouse IgG1/Biotin control at a similar concentration., 5. Binding was blocked when reagent was pre incubated with a 10-fold excess of C2549-22R. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.