Anti-CD104 (Integrin beta-4, GP150, ITGB4)

CD104 is a 205kD type I transmembrane glycoprotein, known as integrin B4 chain or B4 integrin, that associates with integrin a6 (CD49f) forms a6/B4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: <2ug/10e5 cells in 100ul, Immunohistochemistry: acetone-fixed frozen, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.