Anti-CD104 (Integrin beta4), clone 2Q793

Applications: , Suitable for use in Immunohistochemistry, Flow Cytometry, Immunoprecipitation and Inhibition of Cell Attchment. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. , Flow Cytometry: 0.5ug detects human integrin beta4 in 1x10e6 A431 cells. 0.5ug detects integrin beta4 in 1x10e6 HeLa cells. Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. , Inhibition of Cell Attachment: Induces a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40% of the wound are 120 hours post wounding. These results demonstrate that the beta4 adhesion-blocking antidody may have adverse effects on normal tissue (Egles, C, et al., 2010), Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ,