Anti-CD104 (Integrin beta4), clone 2Q792

Applications: , Suitable for use in Flow Cytometry, Immunoprecipitation, Inhibition of cell attachment. Other applications not tested. Recommended Dilution: Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin. , Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. , Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. , Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. , Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.