Anti-APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1,

Alternate Name: APEX, APEX1, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic), Domain Information: Exonuclease III [DNA replication, recombination, and repair] , Gene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity, uracil DNA N-glycosylase activity, endodeoxyribonuclease activity, transcription co-repressor activity, 3'-5' exonuclease activity, endonuclease activity, DNA binding, transcription co-activator activity, base-excision repair, DNA repair, transcription from Pol II promoter, obsolete, nucleus, Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: ELISA: 1:32000, Western blot: 0.2-1ug/ml. ~35kD band seen in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1, NP_542380.1.. Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A2298-74N-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.