Kanamycin Sulfate Solution (100×)

Background: Kanamycin is an aminoglycoside antibiotic that binds to 30S ribosomes to cause misreading of mRNA codes, thereby inhibiting protein synthesis. It is effective against both Gram-positive bacteria and Gram-negative bacteria. Kanamycin can be used in cell culture to inhibit bacterial contamination. In molecular biology research, it is often used to selectively screen bacterial clones that have successfully transformed the kanamycin resistance gene. It is also commonly used in agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying npt II (APH3) genes. General Information: Product Form: Liquid Concentration: 100x Kanamycin Sulfate: 10mg/mL Recommended working concentration: 30-100µg/mL Dissolvent: 0.85% NaCl Antimicrobial spectrum: Gram-positive bacteria & Gram-negative bacteria Storage: -20 °C, Shading light Shipping: Ice bag or dry ice Expiration date: 12 months. Notes 1. This product is for research use only. 2. This product is sterilized by 0.22 µm filtration. 3. It is necessary to pay attention to the aseptic operation and avoid the pollution. 4. Avoid repeated freezing and thawing. It is recommended to defrost at 2-8°C and aliquot the solution and store at -20 °C. 5. This product is a concentrated solution. Please dilute it as needed.