HHBS [Hanks' Buffer with 20 mM Hepes]

Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation such as calcium, membrane potential assay buffer preparation.