Strep-Tactin(R) TACS Agarose Column

Strep-Tactin(R) TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin(R). A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS(R)/Nano-TACS(R)). In this approach, Fab fragments fused to a Twin-Strep-tag(R) (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin(R) on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin(R) Agarose by biotin addition. This way the cells are eluted ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS(R) for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin(R) TACS Agarose columns also suitable for negative cell selection or depletion approaches.