NZYGelpure

NZYGelpure kit is designed for the purification of DNA from TAE/TBE agarose gels and for direct purification of PCR products. Average recoveries range from 60 to 90% depending on the fragment size. NZYGelpure purification kit utilizes a silica-gel based membrane which selectively adsorbs up to 20 µg of DNA fragments in the presence of specialized binding buffers. Soluble agarose, nucleotides, oligos (<30-mer), primer dimers, enzymes, mineral oil and other impurities do not bind to the membrane and are washed away. DNA fragments are then eluted off the column and can be used for downstream protocols without further processing. Binding Buffer contains a pH indicator, allowing the evaluation of optimal pH for DNA binding. The pH indicator does not interfere with DNA binding.High quality, purified DNA using NZYGelpure:The capacity of NZYGelpure to recover DNA fragments of different sizes from an agarose gel was tested by comparing with a similar competitor product. Thus, two different PCR products of 2 and 3 kb, respectively, were separated (in duplicate) through electrophoresis on a 1% (w/v) agarose gel and then purified from gel using NZYGelpure and the competitor kit, following the respective manufacture protocols. A260/A280: <1.8 Elution volume: 30-50 µL Binding capacity: 20 µg. Components: Binding BufferWash Buffer (concentrate)Elution Buffer (does not contain EDTA)NZYTech Spin ColumnsCollection Tubes (2 mL). Shipping Conditions: Room Temperature. Storage Conditions: 15 °C to 25 °C. Application: Cloning, PCR, Sequencing. Starting Material: DNA from agarose gels, DNA from enzymatic reactions. Scale: Miniprep. Features: Manual, pH indicator for DNA binding. Format: Column. Product Category: Nucleic Acids Clean-Up. Isolation Technology: Silica membrane. Protocol Time: Approx. 15 min