Rat Peroxidase Anti-Peroxidase (PAP) Soluble Complex

This product was prepared from rat ascites by delipidation, salt fractionation and ion exhange chromatography.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Peroxidase. The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rat, the PAP complex can be utilized in any assay that utilizes a rat primary antibody. With the addition of a secondary antibody that recognizes rat, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).