Wnt-11, Recombinant, Human (Protein Wnt-11, Wnt11)

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation. Mature human Wnt-11 shares 98% aa sequence identity with mouse, rat and bovine Wnt-11, and 99%, 96% and 88% aa identity with canine, equine and chick Wnt-11, respectively. Mouse Wnt-11 is expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium. It is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters. It is also expressed in several human cancers. In vitro, Wnt-11 has been shown to induce cardiomyocyte differentiation from unfractionated mouse bone marrow mononuclear cells, and to promote hematopoietic differentiation from human embryoid body stem cells by engaging Frizzled-7 in the presence of E-cadherin. In general, there are three signaling pathways associated with Wnt-receptor interaction. The canonical pathway results in b-Catenin accumulation and TCF/LEF-1- mediated gene transcription. Non-canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. Wnt-11 often, but not exclusively, signals by a non-canonical pathway and may repress canonical signaling by other Wnts. However, when complexed with Wnt-5a, Wnt-11 can also enhance canonical signaling. Recombinant protein corresponding to aa25-354 of human wNT-11 expressed in CHO cells (NP_004617). , Activity: Measured in a cell proliferation assay using IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is typically 0.6-2.4ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.