SPARC-like 1, Recombinant, Mouse, aa17-650, His-Tag

SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause anti adhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen, in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Source: Recombinant protein corresponding to aa17-650 from mouse SPARC-like 1 fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~71.7kD (642aa), Endotoxin: ~1EU/1ug (LAL), AA Sequence: IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLFLEHHHH HH, Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.