Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His) (recombinant human)

Activity: Immobilized Human SIRPA-6His(Cat#PKSH033882) at 10µg/ml (100 µl/well) can bind Anti-Human SIRPA mAb-Fc. The ED50 of Anti-Human SIRPA mAb-Fc is 15.1 ng/ml. Protein Construction: Recombinant Human Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His tag at the C-terminus. Sequence: Glu31-Arg370. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 45-60 kDa. Protein function: Signal Regulatory Protein alpha (SIRPalpha) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPalpha contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPalpha can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPalpha acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPalpha shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPalpha engagement generally produces a negative regulatory signal, it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation Protein function: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [The UniProt Consortium]