MAP Kinase 2, Recombinant, Mouse, Unactive (Mitogen-activated Protein Kinase 2, MAPK 2, MAPK2, Extra

An important convergence point involved in the signal transduction pathways of many different growth factors, hormones, and cytokines is a family of 41-44kD serine/threonine kinases collectively called either MAPKs (for mitogen-activated protein kinases) or Erks (for extracellular-regulated kinases). MAPK is activated by sequential phosphorylation on both tyrosine and threonine residues by the dual tyrosine/threonine kinase MEK (MAPK kinase). The serine/threonine kinase Raf can phosphorylate and activate MEK, in turn, during intracellular signaling. Activation of MAPK is directly regulated by a specific MAPK phosphatase and indirectly regulated by protein kinase A activation which results in inhibition of Raf activity in mammalian cells. Source Unactive, recombinant full-length mouse p42 MAP Kinase 2/Erk2 fused to GST-Tag at N-terminus, expressed in E.coli. , Specific Activity: The enzyme preparation demonstrates no endogenous kinase activity. This product demonstrates <1% of maximum activity. Activated by phosphorylation with MEK1 , Activation Assay: 2uM unactive MAP Kinase 2 was activated using 0.4uM active MEK1, diluted 100-fold, and the increased activity against MBP determined. The activation and assay are described in the Protocol section. MS Tryptic Fingerprint: Confirmed identity as MAP Kinase 2 with the translated native sequence listed in the Protocol section. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.