IL13RA2 / CD213A2 Protein (His & Fc tag) (recombinant mouse)

Activity: 1. Immobilized human IL13 at 20 µg/ml (100 µl/well) can bind mouse IL13RA2 with a linear ranger of 0.5-2 µg/ml.2. Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/ml. Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Lys 334) of mouse IL13Ra2 (NP_032382.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Sequence: Met 1-Lys 334. Fusion tag: C-His-Fc Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. Apparent Molecular Mass: 75-80 kDa. Protein function: Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Ralpha2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Ralpha2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Ralpha2 receptor in a manner that cannot be differentiated from the interaction of wild-type viru Protein function: Binds as a monomer with high affinity to interleukin-13 (IL13). [The UniProt Consortium]