HIV Integrase p31, Recombinant (Human Immunodeficiency Virus)

ntegrase is an enzyme, a product of the HIV1 Gag/Pol gene, the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32kD and is composed of three domains. No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic. Source: Recombinant corresponding to HIV-1 immunodominant regions from integrase p31 (aa9-289) fused to 6-His-tag expressed in E. coli. , Applications:, Suitable for use in ELISA, Lateral Flow and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.