Cyclin H, Recombinant, Human (Cyclin-H, CCNH, MO15-associated Protein, p34, p37)

Cyclin H is a member of the cyclin-dependent protein kinase family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). Cyclin H is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. Recombinant human Cyclin H, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-323 of human Cyclin H 6x His tagged expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD, IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL, Applications: Suitable for use in Western Blot. Other applications not tested. Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.