CD4 (V1-V2), Recombinant, Human (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growt

Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V2 domains of CD4 (MW approx: 23kD). Binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA. , Biological Activity: , This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs. Applications: Suitable for use in ELISA, Western ELISA, Neutralization, and Drug screening assays. Other applications not tested. Recommended Dilution: ELISA: 10-100ng , Western Blot: 10-100ng , Neutralization: 1-10ug/ml , Drug screening: 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate or directly using HIV-1 rgp120-peroxidase conjugate. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.