BRD3 bromodomains 1 and 2 (human, recombinant)

Bromodomain-containing protein 3 (BRD3) is a member of the bromodomain and extra-terminal domain (BET) family. It is composed of two N-terminal bromodomains (BD1 and BD2) that bind acetylated lysine on histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, and an extra-terminal domain that mediates chromatin interactions. BRD3 is ubiquitously expressed and localizes to the nucleus where it preferentially binds acetylated histone H4 lysine 5 (H4K5), H4K12, and H4K14 at active transcription sites. Knockdown of Brd3 enhances differentiation of C2C12 skeletal muscle myoblasts. Chromosomal translocations leading to the fusion of BRD3 with the nuclear protein in testis (NUT) gene and resulting in expression of BRD3-NUT fusion proteins are associated with NUT midline carcinoma (NMC). Cayman's BRD3 bromodomains 1 and 2 protein can be used for Western blot (WB).Synonyms: Bromodomain containing protein 3, ORFX, RING3L, RING3-like protein. Purity: >85% (estimated by SDS-PAGE). Source: Recombinant human N-terminal GST-tagged BRD3 bromodomains 1 and 2 expressed in E. coli. Amino Acids: 2-434. MW: 75 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol. Storage Buffer: 50 mM Tris, pH 8.0, with 150 mM sodium chloride and 20% glycerol. Applications: WB. UniProt Accession #: Q15059.