Arylsulfatase A / ARSA Protein (His tag) (recombinant mouse)

Activity: Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100 pmoles/min/µg. Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ser 506) of mouse ARSA (NP_033843.2) precursor was expressed with a C-terminal polyhistidine tag. Sequence: Met 1-Ser 506. Fusion tag: C-His Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. Apparent Molecular Mass: 60 kDa. Protein function: Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate re Protein function: Hydrolyzes cerebroside sulfate. [The UniProt Consortium]