Anti-SETDB1 (human, recombinant)

Histone-lysine N-methyltransferase SETDB1 is a member of the SUV39-related protein lysine methyltransferase (PKMT) family and is involved in epigenetic regulation. It is composed of an N-terminal domain that contains two nuclear export signals, two nuclear localization signals, three tudor domains that facilitate complexation with transcriptional regulators, and a methyl-CpG-binding domain (MBD), which binds to DNA, and a C-terminal domain that contains pre-SET, SET, and post-SET domains, which are responsible for the methyltransferase activity. Alternative splicing of SETDB1 produces two shorter isoforms with either a truncated post-SET domain, which retains enzymatic activity, or a full C-terminal truncation, which lacks enzymatic activity. SETDB1 is ubiquitously expressed and localizes to the nucleus and cytoplasm. It induces gene silencing by di- and trimethylation of histone H3 at lysine 9 (H3K9) using S-adenosylmethionine as the methyl donor and regulates various cellular processes, including cell division and proliferation, retroelement suppression, immune cell function, X chromosome inactivation, nervous system development, and the formation of promyelocytic leukemia nuclear bodies (PML-NBs). Overexpression of SETDB1 increases the number of colon cancer cells in the S and G2/M phases in vitro and promotes tumor growth in vivo. Increased SETDB1 protein levels are associated with schizophrenia, and mutations in SETDB1 have been found in patients with inflammatory bowel disease (IBD). Cayman's SETDB1 (human, recombinant) protein can be used for enzyme activity assays.Synonyms: ERG-associated Protein With SET domain, ESET, Histone-lysine N-methyltransferase SETDB1, SET Domain Bifurcated 1, KMT1E, Histone H3K9 Methyltransferase 4, H3K9-HMTase 4. Purity: >80% estimated by SDS-PAGE. Source: Active recombinant human N-terminal His-GST-tagged SETDB1 expressed in insect cells. Amino Acids: 510-1,290. MW: 114 kDa. Formulation: (Request formulation change), 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 20% glycerol, and variable glutathione. Host: Insect cells. UniProt Accession #: Q15047.