14-3-3 alpha/beta protein-GST fusion

Recombinant full-length human 14-3-3alpha/beta was expressed in E.coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry. Protein function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. [The UniProt Consortium]