Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen . In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

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IL8, Human interleukin 8, Real Time PCR Primer Set
IL8, Human interleukin 8, Real Time PCR Primer Set

Artikelnummer: VHPS-4563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: NAF, IL8, IL-8, MDNCF, LYNAP, NAP-1, GCP-1, CXCL8, MONAP, MDNCF-a, MDNCF-b, MDNCF-c, Emoctakin, IL-8(5-77), IL-8(6-77),...
Anwendung: RNA quantification
43,00 €
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Actb, Mouse actin, beta, Real Time PCR Primer Set
Actb, Mouse actin, beta, Real Time PCR Primer Set

Artikelnummer: VMPS-96

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Actb, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Anwendung: RNA quantification
43,00 €
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Il6, Mouse interleukin 6, Real Time PCR Primer Set
Il6, Mouse interleukin 6, Real Time PCR Primer Set

Artikelnummer: VMPS-3096

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Il6, mCG_11634, Interleukin 6, Interleukin 6, isoform CRA_b
Anwendung: RNA quantification
43,00 €
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Human MEGImmune Primer Library
Human MEGImmune Primer Library

Artikelnummer: HMEGIMM

Masterplate for SYBR green based Real Time PCR. Contains 374 primer sets directed against human calcium regulation related genes and 10 housekeeping gene primer sets. Provided in a 96-well microplate (10 µl - 10 µM). Perform up to 100 PCR arrays (based on 10 µl assay volume per reaction). Just add cDNA template and...
Schlagworte: HMEGIMM
Anwendung: Gene expression profiling, PCR array
2.924,00 €
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Human MEGoptosis Primer Library
Human MEGoptosis Primer Library

Artikelnummer: HMEGOP-1

Masterplate for SYBR green based Real Time PCR. Contains 374 primer sets directed against human calcium regulation related genes and 10 housekeeping gene primer sets. Provided in a 96-well microplate (10 µl - 10 µM). Perform up to 100 PCR arrays (based on 10 µl assay volume per reaction). Just add cDNA template and...
Schlagworte: HMEGOP-I
Anwendung: Gene expression profiling, PCR array
2.924,00 €
Bewerten
18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set
18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set

Artikelnummer: VHPS-10350

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: qPCR, gene expression analysis
Anwendung: RNA quantification
43,00 €
Bewerten
B2M, Mouse beta-2 microglobulin, Real Time PCR Primer Set
B2M, Mouse beta-2 microglobulin, Real Time PCR Primer Set

Artikelnummer: VMPS-563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: B2m, Beta-2 microglobulin
Anwendung: RNA quantification
43,00 €
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HCRTR1, Human hypocretin (orexin) receptor 1, Real Time PCR Primer Set
HCRTR1, Human hypocretin (orexin) receptor 1, Real Time...

Artikelnummer: VHPS-4015

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Ox1R, Ox1-R, Ox-1-R, HCRTR1, Orexin receptor type 1, Hypocretin receptor type 1
Anwendung: RNA quantification
43,00 €
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HCRTR2, Human hypocretin (orexin) receptor 2, Real Time PCR Primer Set
HCRTR2, Human hypocretin (orexin) receptor 2, Real Time...

Artikelnummer: VHPS-4016

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Ox2R, Ox2-R, Ox-2-R, HCRTR2, Orexin receptor type 2, Hypocretin receptor type 2
Anwendung: RNA quantification
43,00 €
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IL1B, Human interleukin 1, beta, Real Time PCR Primer Set
IL1B, Human interleukin 1, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-4531

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: IL1B, IL1F2, IL-1 beta, Catabolin, Interleukin-1 beta
Anwendung: RNA quantification
43,00 €
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VEGF, Human, Real Time PCR Primer Set
VEGF, Human, Real Time PCR Primer Set

Artikelnummer: VHPS-9899

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: VPF, VEGF, VEGFA, VEGF-A, Vascular permeability factor, Vascular endothelial growth factor A
Anwendung: RNA quantification
43,00 €
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Rat Housekeeping Genes Primer Sets
Rat Housekeeping Genes Primer Sets

Artikelnummer: RHK-1

Includes primer sets for Actb, B2m, Gapd, Gusb, Hprt1, Pgk, Pp1a, Rpl13a, Tbp and Tfrc. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
138,00 €
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