ent-8-iso-15(S)-Prostaglandin F2alpha-d9

ent-8-iso-15(S)-Prostaglandin F2alpha-d9 (ent-8(S)-15-epi-PGF2alpha-d9) contains nine deuterium atoms at the 17, 17', 18, 18', 19, 19', 20, 20, and 20 positions. It is intended for use as an internal standard for the quantification of ent-8-iso-15(S)-PGF2alpha by GC- or LC-mass spectrometry. Isoprostanes are produced by the non-enzymatic, free radical peroxidation of phospholipid-esterified arachidonic acid. They have been used as biomarkers of oxidative stress, but they also have been found to have potent biological activity. ent-8-iso-15(S)-PGF2alpha is a potent vasoconstrictor of porcine retinal and brain microvessels with EC50 values of 15 and 24 nM, respectively. This isoprostane is about ten-fold more potent than 8-iso-PGF2alpha in a whole blood platelet aggregation inhibition assay.