Dihydroethidium

Dihydroethidium is a cell-permeable blue fluorescent dye that upon entering cells interacts with superoxide to form oxyethidium, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It displays excitation/emission spectra of 490/590 nm. This selective and sensitive probe has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells.Formal Name: 5-ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine. CAS Number: 104821-25-2. Synonyms: Hydroethidine, PD-MY 003. Molecular Formula: C21H21N3. Formula Weight: 315.4. Purity: >98%. Emission: 590 nm. Excitation: 490 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.5 mg/ml, DMSO: 10 mg/ml, DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml, Ethanol: 0.25 mg/ml. lambdamax: 224, 271, 354 nm. SMILES: NC1=CC2=C(C3=C(C=C(N)C=C3)N(CC)C2C4=CC=CC=C4)C=C1. InChi Code: InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3. InChi Key: XYJODUBPWNZLML-UHFFFAOYSA-N.