Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)*

Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)*
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
ABD-17016 25 nmol -

3 - 8 Werktage*

112,00 €
 
The biotin-modified deoxyuridine 5'-triphosphates are widely used for a variety of... mehr
Produktinformationen "Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)*"
The biotin-modified deoxyuridine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3'-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dUTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '11' is the number of carbon atoms in the backbone of the linker between dUTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dUTP into DNA. It is suggested the length of linker '11' is optimal for the majority of applications. Bio-11-dUTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells and tissues.
Hersteller: AAT Bioquest
Hersteller-Nr: 17016

Eigenschaften

Anwendung: Labeling
MW: 928,62 D

Datenbank Information

CAS : 86303-25-5| Finde Alternativen

Handhabung & Sicherheit

Lagerung: -20°C
Versand: -20°C (International: -20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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