5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm, emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.Formal Name: 5-cyano-2,3-bis(4-methylphenyl)-2H-tetrazolium, monochloride. CAS Number: 90217-02-0. Synonyms: CTC, Cyanotolyl Tetrazolium Chloride. Molecular Formula: C16H14N5 . Cl. Formula Weight: 311.8. Emission: 590 nm. Excitation: 510-560 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.1 mg/ml, DMSO: 0.25 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 299 nm. SMILES: CC(C=C1)=CC=C1N2N=C(C#N)N=[N+]2C3=CC=C(C)C=C3.[Cl-]. InChi Code: InChI=1S/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15,/h3-10H,1-2H3,1H/q+1,/p-1. InChi Key: SSJZAXOTLCJNLF-UHFFFAOYSA-M.