TAPSO Free Acid (3-[N-tris-(hydroxymethyl) methylamino]-2- hydroxypropanesulfonic acid)

TAPSO Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes. Synonyms: 2-Hydroxy-3-[tris(hydroxymethyl)methylamino]-1-propanesulfonic acid, N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid, CAS Number: 68399-81-5, Molecular Formula: C?H??NO?S, Molecular Weight: 259.28, Appearance: White crystalline powder, Purity: >99%, Solubility (0.1M, dH2O): Clear, colorless and complete, Water (KF): <1%, pH (1%): 4.7-5.3, Absorbance (A280) (1M dH2O): <0.02, Absorbance (A260) (1M dH2O): <0.04, DNase: None detected, RNase None detected, Protease: None detected