Ursodeoxycholic Acid-d4 MaxSpec(R) Standard

Ursodeoxycholic acid-d4 (UDCA-d4) is intended for use as an internal standard for the quantification of UDCA (Cay-15121) by GC- or LC-MS. UDCA is a secondary bile acid formed via epimerization of chenodeoxycholic acid (CDCA, Cay-10011286). UDCA is also a metabolite of lithocholic acid (LCA, Cay-20253) in human liver microsomes. It inhibits taurocholic acid (Cay-16215) uptake in HeLa cells expressing recombinant sodium/taurocholate cotransporting polypeptide (NTCP) with an IC50 value of 3.6 µM. UDCA (50 µM) inhibits apoptosis induced by deoxycholic acid (DCA, Cay-20756, Cay-18231) or ethanol in primary rat hepatocytes. Dietary administration of UDCA blocks DCA-induced increases in the number of TUNEL-positive hepatocytes in rats. Formulations containing UDCA have been used in the treatment of primary biliary cirrhosis. Ursodeoxycholic Acid-d4 MaxSpec(R) standard is a quantitative grade standard of Ursodeoxycholic Acid-d4 (Cay-21892) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This Ursodeoxycholic Acid-d4 MaxSpec(R) standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.Formal Name: (3alpha,5beta,7beta)-3,7-dihydroxy-cholan-24-oic-2,2,4,4-d4 acid. CAS Number: 347841-46-7. Synonyms: UDCA-d4. Molecular Formula: C24H36D4O4. Formula Weight: 396.6. Purity: >95%. Formulation: (Request formulation change), A solution in methanol at 100 µg/ml. SMILES: OC(CC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@@H](O)C[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C)=O. InChi Code: InChI=1S/C24H40O4/c1-14(4-7-21(27)28)17-5-6-18-22-19(9-11-24(17,18)3)23(2)10-8-16(25)12-15(23)13-20(22)26/h14-20,22,25-26H,4-13H2,1-3H3,(H,27,28)/t14-,15+,16-,17-,18+,19+,20+,22+,23+,24-/m1/s1/i8D2,12D2. InChi Key: RUDATBOHQWOJDD-QGCKUCIHSA-N.