Tween 20

Tween(R)-20 (polysorbate 20) is a polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays. Tween(R)-20 is typically included in blocking buffers and washing buffers at a concentration of 0.1% to reduce non-specific antibody binding. TWEEN is a registered trademark of Uniqema Americas LLC.