TMB

TMB is a colorimetric substrate for peroxidases, including hemoglobin, myeloperoxidase, and horseradish peroxidase (HRP). Enzymatic oxidation of TMB produces a radical cation followed by a diimine product that can be quantified by colorimetric detection at 652 and 450 nm, respectively, as a measure of peroxidase activity. TMB has commonly been used as a substrate for HRP in ELISA and immunohistochemical applications.Formal Name: 3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine. CAS Number: 54827-17-7. Synonyms: 3,3',5,5'-Tetramethylbenzidine. Molecular Formula: C16H20N2. Formula Weight: 240.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 20 mg/ml, DMSO: 5 mg/ml. lambdamax: 212, 288 nm. SMILES: NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C. InChi Code: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3. InChi Key: UAIUNKRWKOVEES-UHFFFAOYSA-N.