Ac-IETD-AFC (trifluoroacetate salt)

Ac-IETD-AFC is a fluorogenic substrate that can be cleaved by caspase-8 and related enzymes that recognize the amino acid sequence IETD, including caspase-3 processing enzyme, caspase-10, and granzyme B. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name: N-acetyl-L-isoleucyl-L-alpha-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine, trifluoroacetate salt. Synonyms: N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin. Molecular Formula: C31H38F3N5O12 . XCF3COOH. Formula Weight: 729.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml. lambdamax: 232, 340 nm. SMILES: OC(C[C@H](NC(C(NC([C@@H](NC([C@H]([C@@H](C)CC)NC(C)=O)=O)CCC(O)=O)=O)[C@@H](C)O)=O)C(NC(C=C1O2)=CC=C1C(C(F)(F)F)=CC2=O)=O)=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C31H38F3N5O12.C2HF3O2/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16,3-2(4,5)1(6)7/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45),(H,6,7)/t13-,14+,19-,20-,25-,26?,/m0./s1. InChi Key: YWNQGRLHYRWGIP-FKXNOZCSSA-N.