ZsGreen/Luciferase Safe-Harbor HEK293 Cell Line

Recombinant stable HEK293 cell line constitutively expressing ZsGreen (bright green fluorescent protein derived from Zoanthus sp. reef coral) and firefly luciferase, which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19 using CRISPR/Cas9. Expression of ZsGreen and the luciferase reporter are driven by a EF1A promoter (Figure 1). Cells were selected by limiting dilution to obtain a monoclonal population. The lack of random integration of ZsGreen/luciferase into the HEK293 genome guarantees this cell line behaves like parental HEK293 cells.