WM9 Viable Cells

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details. WM9 is a human metastatic melanoma cell line that was established from a metastatic site (left axillary node) in a male patient. This cell line features the V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN hemizygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM9 cells produce xenograft tumors when injected into immunocompromised mice.