WM1862 Viable Cells

Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details. WM1862 is a non-tumorigenic (RGP) primary human melanoma cell line without metastatic capability. This cell line displays pigmented, mesenchymal and spherical morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. These cells expresses wild type N-RAS, c-KIT, and CDK4. WM1862 cells produce xenograft tumors when injected into immunocompromised mice.