WM1862 Non-Viable Cell Pellet

Cells were grown sub-confluent to approximately (2x10^6) cells in Tumor specialized media and then trypsinized. Cells were harvested and cell pellets were flash frozen and stored at -80°C. Non-viable cell pellet was generated from cell line WM1862. WM1862 is a non-tumorigenic (RGP) primary human melanoma cell line without metastatic capability. This cell line displays pigmented, mesenchymal and spherical morphology. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. These cells expresses wild type N-RAS, c-KIT, and CDK4. WM1862 cells produce xenograft tumors when injected into immunocompromised mice.