WM1341D Non-Viable Cell Pellet

Bulk, (on demand), cells will be grown to requested amount. Cells will be grown sub-confluent in Tumor specialized media, trypsinized, flash frozen, and stored at -80°C. Non-viable cell pellet was generated from cell line WM1341D. WM1341D is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display epithelial square and triangular morphology in culture. This cell line features the specific V600R (Val600Arg) mutation at codon 600 in the BRAF gene. The V600R mutation results in an amino acid substitution at position 600 in BRAF, from a valine (V) to an arginine (R). This mutation occurs within the activation segment of the kinase domain. Mutations at V600 result in increased kinase activity and are transforming in vitro. WM1341D cells produce xenograft tumors when injected into immunocompromised mice.