Multiple Tag Cell Lysate

Multiple Tag is a specially designed fusion protein for use as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags from N-terminus to C-terminus in the order as shown in the table:, Tag Sequence Trx MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY , QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL, KEFLDANLA, T7 CMASMTGGQQMG, HSV QPELAPEDPEDC, c-Myc EQKLISEEDLC, VSV-g YTDIEMNRLGK , Glu-Glu CEEEEYMPME, V5 GKPIPNPLLGLDST, E-tag GAPVPYPDPLEPR, Flag-tag DYKDDDDK, S-tag KETAAAKFERQHMDS, HA YPYDVPDYAC, KT3 KPPTPPPEPET, E2 GVSSTSSDFRDR, AU1 DTYRYI, AU5 TDFYLK, 6x His HHHHHH , Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.04-0.2ul per lane of minigel. For accurate loading, dilute with PBS., Optimal dilutions to be determined by the researcher. Storage and Stability: Store lyophilized powder at -20°C or lower. Reconstitute by adding sterile dH2O. Aliquot reconstituted product to avoid repeated freezing and thawing, and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.