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A431 Pervanadate Control Lysate
Artikelnummer: A0001-52B.100
Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by Western blotting using anti-Phosphotyrosine. Confluent cultures of A431...
| Anwendung: | WB |
341,00 €
A431 Cells, EGF-Stimulated, Lysate
Artikelnummer: A0001-55.100
Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. Recommended Dilutions: Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. This preparation may be...
| Anwendung: | WB |
545,00 €
Akt Control Cell Extracts (Rac PKa, PKBa),
Artikelnummer: A1124-04.10
Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid...
| Schlagworte: | Akt, PKB, Akt1, PKB alpha, EC=2.7.11.1, AKT1 kinase, RAC-PK-alpha, Protein kinase B |
462,00 €
IkappaB alpha Control Cell Extracts,
Artikelnummer: I2500-01B.200
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins (1-3). Activation occurs via phosphorylation of IkB-a at Ser32 and Ser36, resulting in the release and nuclear translocation of active NF-kB (3-7). IkB-a phosphorylation and resulting...
| Schlagworte: | IKBA, NFKBIA, IkB-alpha, IkappaBalpha, I-kappa-B-alpha, NF-kappa-B inhibitor alpha, Major histocompatibility complex... |
| Anwendung: | WB |
462,00 €
Jurkat Pervanadate Control Lysate
Artikelnummer: J9000-01.100
Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of Jurkat...
| Anwendung: | WB |
341,00 €
Jurkat+ Pervanadate Lysate
Artikelnummer: J9000-01A.100
Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of Jurkat...
| Anwendung: | WB |
341,00 €
Multiple Tag Cell Lysate
Artikelnummer: M3889-50.200
Multiple Tag Cell Lysate is whole E. coli cell lysate containing about 30% of a specially designed fusion protein as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags in the order as shown in the table:, Tag Sequence Trx MSDKIIHLTD DSFDTDVLKA...
| Anwendung: | Dot Blot, WB |
375,00 €
Multiple Tag Cell Lysate
Artikelnummer: M3889-51.200
Multiple Tag is a specially designed fusion protein for use as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags from N-terminus to C-terminus in the order as shown in the table:, Tag Sequence Trx MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA...
| Anwendung: | Dot Blot, WB |
418,00 €
SAPK1, JNK (Stress Activated Protein Kinase, Jun Kinase)...
Artikelnummer: S0096-04F.10
The SAPK/JNK pathway is homologous to the MAPK pathway in its overall form but is activated by largely distinct stimuli (1). A variety of extracellular stimuli activate the JNK/SAPK pathway including inflammatory cytokines, UV light, inhibitors of protein synthesis and osmotic stress (1-5). Inflammatory cytokines...
462,00 €
HEK293 Whole Cell Lysate MG-132 Treated
Artikelnummer: W09-001-GV8
HEK293 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM...
| Anwendung: | WB |
| Wirt: | Human |
| Spezies-Reaktivität: | human |
302,00 €
HeLa Whole Cell Lysate MG-132 Treated
Artikelnummer: W09-001-GV9
HeLa cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium...
| Anwendung: | WB |
| Wirt: | Human |
| Spezies-Reaktivität: | human |
302,00 €
KG-1 Whole Cell Lysate
Artikelnummer: W09-001-GY6
Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot...
| Anwendung: | WB |
| Wirt: | Human |
| Spezies-Reaktivität: | human |
204,00 €