TNFSF13/APRIL BioAssay(TM) ELISA Kit (Monkey)

Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation- inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Monkey TNFSF13/APRIL. Sensitivity: <10pg/ml, Range: 156pg/ml-10,000pg/ml, Specificity: Recognizes natural and recombinant Monkey TNFSF13/APRIL. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Monkey TNFSF13/APRIL BioAssay(TM) ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey TNFSF13/APRIL has been precoated onto 96-well plates. Standards(Expression system for standard: NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey TNFSF13/APRIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey TNFSF13/APRIL amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Monkey TNFSF13 antibody, 1x plate, Lyophilized recombinant Monkey TNFSF13 standard, 2x10ng/tube, Biotinylated anti-Monkey TNFSF13 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.