TGF-Beta 2 BioAssay(TM) ELISA Kit (Equine)

TGF-Beta 2 BioAssay(TM) ELISA Kit (Equine)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
396777.96 96 tests - -

3 - 19 Werktage

704,00 €
Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that... mehr
Produktinformationen "TGF-Beta 2 BioAssay(TM) ELISA Kit (Equine)"
Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured horse equine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Equine TGF-beta 2. Sensitivity: <10pg/ml, Range: 31.2pg/ml-2000pg/ml, Specificity: Recognizes natural and recombinant Equine TGF-beta 2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Equine TGF-Beta 2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Equine TGF- Beta 2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Equine TGF-Beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine TGF-Beta 2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Equine TGFB2 antibody, 1x plate, Lyophilized recombinant Equine TGFB2 standard, 2x10ng/tube, Biotinylated anti-Equine TGFB2 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: TGFB2
Hersteller: United States Biological
Hersteller-Nr: 396777


Anwendung: ELISA
Wirt: Horse
Reaktivität: Horse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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