Meteorin/METRN BioAssay(TM) ELISA Kit (Mouse)

Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts directly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse Meteorin/METRN. Sensitivity: <12pg/ml, Range: 62.5pg/ml-4000pg/ml, Specificity: Recognizes natural and recombinant Mouse Meteorin/METRN. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse Meteorin/METRN BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Meteorin/METRN has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G22-D291) and test samples are added to the wells, a biotinylated detection specific for Meteorin/METRN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin/METRN amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Metrn antibody, 1x plate, Lyophilized recombinant Mouse Metrn standard, 2x10ng/tube, Biotinylated anti-Mouse Metrn antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.