Lipocalin-2/NGAL BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Human Mouse Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine. Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. The LCN2 gene is located at 9q34 and contains 7 exons. The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22kD. , Assay Range:, 78pg/ml-5, 000pg/ml, Sensitivity: < 10 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P11672, Entrez Gene ID: 16819, Kit Components: 96-well plate pre-coated with anti-Lipocalin-2/NGAL, Mouse, Lyophilized recombinant Lipocalin-2/NGAL, Mouse standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.