Latanoprost ELISA Kit

Latanoprost is 13,14-dihydro-17-phenyl-18,19,20-trinor prostaglandin F2alpha isopropyl ester (13,14-dihydro-17-phenyl-18,19,20-trinor PGF2alpha isopropyl ester), an F-series prostaglandin analog which has been approved for use as an ocular hypotensive drug. Prostaglandin esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost (Latanoprost free acid (Lat-FA), or PhXA 85) is 200 times more potent than the isopropyl ester as an FP receptor ligand tested using the recombinant human FP receptor. Although the exact mechanism of the intraocular hypotensive effect of prostaglandins is unknown, it correlates very closely with the FP receptor ligand binding affinity of FP receptor agonists. The Cayman Latanoprost ELISA is a sensitive detection method for measuring the free acid form of Latanoprost. It also detects the isopropyl ester form of Latanoprost, but with less sensitivity. The assay is most appropriate for use when only one of the two forms is present. Samples containing mixtures of both the isopropyl ester and free acid should be purified and the two compounds separated prior to ELISA analysis.Synonyms: Latanoprost EIA Kit, 17-phenyl-13,14-dihydro trinor Prostaglandin F2alpha, 17-phenyl-13,14-dihydro trinor Prostaglandin F2alpha isopropyl ester, PhXA 85. Assay Range: 3.9-500 pg/ml (free acid) and 15.6-2,000 pg/ml (ester). Sensitivity: 80% B/B0: 15.5 pg/ml (free acid) and 43 pg/ml (ester). Mid-point: 50% B/B0: 50-80 pg/ml (free acid) and 160-300 pg/ml (ester). Cross Reactivity: (Latanoprost (free acid)) 100%, (5-trans Latanoprost) 25.4%, (Latanoprost) 25%, (17-phenyl Prostaglandin F2alpha) 3%, (17-phenyl Prostaglandin F2alpha ethyl amide) 0.70%, (15-epi Latanoprost) 0.16%, (15-keto Latanoprost) 0.10%, (Fluprostenol) <0.01%, (Fluprostenol isopropyl ester) <0.01%, (2,3-dinor Fluprostenol) <0.01%, (9-keto Fluprostenol) <0.01%, (11-keto Fluprostenol) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (Prostaglandin D2) <0.01%, (Prostaglandin E2) <0.01%, (Prostaglandin E2 Ethanolamide) <0.01%, (6-keto Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (Prostaglandin F2alpha Ethanolamide) <0.01%, (5-trans Prostaglandin F2alpha) <0.01%, (Thromboxane B2) <0.01%, (Unoprostone) <0.01%, (Unoprostone isopropyl ester) <0.01%. Origin: Animal/Mouse, Animal/Rabbit, Animal/Bovine, Animal/Eel.