IL-17C, BioAssay(TM) ELISA Kit (Human) (CX2, Interleukin 17C)

IL-17C, BioAssay(TM) ELISA Kit (Human) (CX2, Interleukin 17C)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352922.96 96 tests - -

3 - 19 Werktage*

669,00 €
 
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is... mehr
Produktinformationen "IL-17C, BioAssay(TM) ELISA Kit (Human) (CX2, Interleukin 17C)"
IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of human IL-17C in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 125-8000pg/ml, Specificity: Natural and recombinant human IL-17C, Test Principle: This human IL-17C BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17C amount of sample captured in plate. Kit Components: 96-well plate precoated with human IL-17C antibody, 1, Lyophilized recombinant human IL-17C standard, 10ng/tube×2, Biotinylated human IL-17C antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: IL17C, IL-17C, Cytokine CX2, Interleukin-17C
Hersteller: United States Biological
Hersteller-Nr: 352922

Eigenschaften

Anwendung: ELISA
Wirt: Human
Reaktivität: Human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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