Histone H3, acetylated, Immunoprecipitation Assay Kit, BioAssay,

For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher using quantitative PCR or Southern slot-blot analysis with promotor specific primers or probe. Applications: immunoprecipitation of chromatin using anti-Acetyl-Histone H3 , , Capacity:, 22 assays , , Components: , Anti-Acetylated Histone H3, ssDNA/Protein A Agarose, all buffers and solutions , Other components required but not included as part of kit are: Reagents Equipment, Cells, stimulated or treated as appropriate for the experimental system, 37% Formaldehyde, PBS, Dry ice, Elution buffer: 1% SDS, 0.1M NaHCO3, Molecular Biology grade Proteinase K, Glycogen or tRNA, 50% Phenol/50%Chloroform (containing 1% isoamyl alcohol), 95% Ethanol, 70% Ethanol, vortex mixer, rotating wheel/platform, shaking incubator, timer, variable volume (5-1000l) pipettes + tips, microfuge, variable temperature water bath, humidified 37ºC incubator, cell scraper, sonicator, Storage and Stability: Stable for 1 year at 4°C from date of shipment.