Heparan Sulfate Proteoglycan (HSPG) BioAssay(TM) ELISA Kit (Rat)

Sample Type:, Serum, plasma, tissue homogenates and other biological fluids, Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HSPG in rat serum, plasma, tissue homogenates and other biological fluids. Sensitivity: 12.7pg/ml, Range: 31.2-2000pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate Proteoglycan (HSPG). No significant cross-reactivity or interference between Heparan Sulfate Proteoglycan (HSPG) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12%, Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to HSPG. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HSPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSPG, biotin-conjugated antibody and, enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSPG in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate , Standard, 2x vial, Detection Reagent A, 1×120ul, Detection Reagent B, 1×120ul, TMB Substrate, 1×9ml, Wash Buffer (30X), 1×20ml, Standard Diluent, 1×20ml, Assay Diluent A, 1×12ml, Assay Diluent B, 1×12ml, Stop Solution, 1x6ml, Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.