Artikelnummer | Größe | Datenblatt | Manual | SDB | Lieferzeit | Menge | Preis |
---|---|---|---|---|---|---|---|
351644.48 | 48 tests | - | - |
3 - 19 Werktage* |
716,00 €
|
||
351644.96 | 96 tests | - | - |
3 - 19 Werktage* |
877,00 €
|
Bestellen Sie auch per E-Mail: info@biomol.com
Größere Menge gewünscht? Bulk-Anfrage
This protein is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. Sample Type: Serum, plasma, cell culture supernates, tissue homogenates, Intended Use: For the quantitative determination of human granulocyte-macrophage colony stimulating factor (GM-CSF) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Sensitivity: 3.9pg/ml, Range: 15.6-1000pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of human GM-CSF. No significant cross-reactivity or interference between human GM-CSF and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells, Standard (Freeze dried), 2x vials, Biotin-antibody (100X), 1x120ul, HRP-avidin (100X), 1x120ul, Biotin-antibody Diluent, 1x15ml, HRP-avidin Diluent, 1x15ml, Sample Diluent, 1x50ml, Wash Buffer (25X), 1x20ml, TMB Substrate, 1x10ml, Stop Solution, 1x10ml, Adhesive Strip (For 96 wells), 4x strips, Instruction manual, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: | CSF, CSF2, GMCSF, GM-CSF, Molgramostin, Sargramostim, Colony-stimulating factor, Granulocyte-macrophage colony-stimulating factor |
Hersteller: | United States Biological |
Hersteller-Nr: | 351644 |
Eigenschaften
Anwendung: | ELISA |
Wirt: | Human |
Spezies-Reaktivität: | human |
Format: | Solid Phase |
Datenbank Information
KEGG ID : | K05427 | Passende Produkte |
UniProt ID : | P04141 | Passende Produkte |
Gene ID | GeneID 1437 | Passende Produkte |
Handhabung & Sicherheit
Lagerung: | vT |
Versand: | +4°C (International: °C) |
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!